The binding energy of a nucleus is the energy that would be needed to split up the nucleus into separate nucleons .
Example
We calculate the binding energy of the He-4 nucleus
This nucleus consists of 2 protons en 2 neutrons
Mass of the 4 particles : 2 x 1.007276 u + 2 x 1.008665 u = 4.031882 u
Mass of the nucleus : 4.002603 u – 2 x 0.00054858 u = 4.00150584 u
Mass defect Δm : 0.03037616 u
Binding energy : E= Δm.c2 = (0.03037616 x 1.66054 x10-27) (2.99792458 x 108)2 = 4.546664 x 10-12 J = 2.8417 x 107 eV = 28.42 MeV
Binding energy per nucleon = 7.10 MeV
Remark : The smaller the mass per nucleon the more stable the nucleus.